Pdf on jul 20, 2017, manvender singh and others published in vitro propagation of stevia rebaudiana bertoni. Stevia is an erect, taproot and perennial herbaceous species that reaches up to 90 cm in height, with small leaves that measure from 3 to 5 cm in length and 1 to 1. The leaves of this plant has a pleasantly sweet and refreshing taste which is educe by diterpene glycosides stevioside and rebaudiosides, a highpotency sweeteners and. It is commonly known as candyleaf, sweetleaf or sugarleaf. However, it is popular as a sugar substitute, and the glycosidal diterpens in the leaves are 30 times sweeter than sugar. Keeping in view the importance of stevia and salinity, present study had been designed to investigate the effect of salinity on biochemical parameters in two stevia genotypes. Qualitative composition of the sgs in in vitro plants was found to be identical to that of intact plants, but their content in the former plants. It is mainly used as a sweetener and flavor enhancer in the food and beverage industry. Stevia rebaudiana is a shrublike plant in the sunflower family that is native to north and south america and is known for its sweet leaves.
Stevia is native to paraguay south america and also called sweet herb of paraguay. Studies on rapid micropropagation of stevia rebaudiana bertoni. In this report, stevioside and steviol were tested for mutagenicity in salmonella typhimurium strains ta98 and ta100 and for chromosomal effects on cultured human lymphocytes. In vitro multiplication of stevia rebaudiana article pdf available in current science 7011. Pdf in vitro propagation of stevia rebaudiana bertoni in. An overview manvender singh, vinod saharan, jyotsna dayma, deepak rajpurohit, yadunandan sen and ajay sharma department of molecular biology and biotechnology, rajasthan collage of agriculture, maharana pratap university of agriculture and technology, udaipur, rajasthan, india. This plant is recorded as having a noncaloric natural sugar, alternative to artificially. Four solvent extracts ethyl acetate, acetone, chloroform and water of stevia rebaudiana leaves were investigated against staphylococcus aureus, salmonella typhi, escherichia coli, bacillus subtilis, aeromonas hydrophila and vibrio cholerae by. In the present study, a protocol was developed for in vitro micropropagation using 6benzylamino purine bap and kinetin kn for the formation of multiple shoot proliferation and indole3acetic acid iaa, indole3butyric acid iba and 1naphthaleneacetic acid naa. Since these activities may be correlated with the presence of antioxidant compounds, leaf and callus extracts of stevia rebaudiana were evaluated for their total phenols, flavonoids content and total antioxidant cap.
Production of steviosides in ex vitro and in vitro grown. S rebaudiana is originally a south american wild plant katayma et al. Adaptation to ex vitro conditions of stevia rebaudiana. In this study, the functioning of antioxidant metabolism and photosynthesis efficiency during the acclimatisation of stevia rebaudiana plants to ex vitro conditions was determined. Stevia has garnered attention with the rise in demand for lowcarbohydrate, lowsugar food alternatives. Nutrient factors affecting in vitro cultivation of stevia. The present study was aimed at the developing an efficient protocol for successful in vitro rooting of stevia shoot fragments and subsequent acclimatization of regenerants to ex vitro conditions. Comparative studies of in vitro and in vivo grown plants and callus of stevia rebaudiana bertoni. An efficient stevia rebaudiana transformation system and. In vitro mineral nutrition for improving growth and.
The study included the effect of ms salt strength and major elements containing medium at different levels on the in vitro growth and development of stevia rebaudiana the best nutrient medium was ms at full salt strength or modified ms major elements at these levels nh4no3 at 1237. Stevia stevia rebaudiana bertoni, with great potential as a natural sweeteners source, has a high content of sweeteners, which are up to 150 times sweeter than sugar, but virtually with no calories. Indian journal of biotechnology vol 11, october 2012, pp 486490 optimization of micronutrients for the improvement of in vitro plant regeneration of stevia rebaudiana bert. Stem cutting propagation for exsitu conservation of. An in vitro and in vivo comparison of the effect of stevia rebaudiana extracts on different cariesrelated variables. Bertoni pourvi jain, sumita kachhwaha and s l kothari. Steviosides were extracted by hydrolysis and esterification, evaporated to dryness and dissolved in methanol for quantitative analysis by high. Stevia rebaudiana sr is often used by the food industry due to its steviol glycoside content, which is a suitable caloriefree sweetener.
In vitro propagation of stevia rebaudiana semantic scholar. Rooting of the in vitro derived shoots could be achieved following subculture onto auxincontaining medium. Pdf feas elarish, north sinai, suez canal universityscu and biotechnology laboratory in north sinai station, desert research centerdrc cairo. Stevia rebaudiana bertoni bertoni, asteraceae asterales, has long been known to the indians, who call it kaa hee sweet herb. Pdf in vitro culture studies on stevia rebaudiana re nata. Stevia rebaudiana is a plant species in the genus stevia of the sunflower family. Rooting of the in vitroderived shoots could be achieved following subculture onto auxincontaining medium. Pdf an efficient protocol for in vitro regeneration of. In vitro antioxidant activities of stevia rebaudiana leaves. A survival rate of 70% was recorded at the hardening phase. In vitro rapid multiplication of stevia rebaudiana. Pdf in vitro culture studies on stevia rebaudiana re. Nutrient factors affecting in vitro cultivation of stevia rebaudiana.
The present study aims at developing a reliable protocol for in vitro propagation and cryostorage shoot tips of stevia rebaudiana using a vitrification method. Further, both in vitro and in vivo studies indicate that these glycosides and the extracts from sr have pharmacological and therapeutic properties, including antioxidant, antimicrobial, antihypertensive, antidiabetic, and anticancer. Stevia stevia rebaudiana bertoni is a perennial shrub belonging to the asteraceae family. An overview find, read and cite all the research you need on researchgate. The aim of this study was to investigate the effects of mineral salts on plant growth and development of stevia shoot cultures. The purpose of the study is to evaluate the antimicrobial and antitumor activities of stevia rebaudiana asteraceae leaf extracts. Characterization and quantification of hydroxycinnamate. To effectively evaluate the whole stevia leaf extract, we.
In vitro micropropagation of stevia rebaudiana bertoni in. In this study, the hydroxycinnamate derivatives of s. The plant was first brought to the attention of the rest of the world by the botanist moises. Singlenode shoot fragments obtained from plants propagated on ms medium were placed onto media supplemented with. Stevia rebaudiana, in vitro propagation introduction the sweet herb of paraguay, stevia rebaudiana is becoming a major source of sweetener with no calorific value. In vitro shoot multiplication of stevia rebaudiana, an. According to the extent of lipid peroxidation, an oxidative stress occurred during the first hours of acclimatisation. Stevia rebaudiana leaves are used as a zerocalorie natural sweetener in a variety of food products in asian countries, especially in japan. In vitro propagation of stevia rebaudiana bert in bangladesh. Comparative studies of in vitro and in vivo grown plants and. Optimization of micronutrients for the improvement of in. The active compounds are steviol glycosides mainly stevioside and rebaudioside, which have 30 to 150 times the sweetness of sugar, are heatstable, phstable, and not fermentable. Since these activities may be correlated with the presence of antioxidant compounds, leaf and callus extracts of stevia rebaudiana were evaluated for their total phenols, flavonoids content and total antioxidant capacity. Stevia rebaudiana bertoni is a medicinal plant belongs to asteraceae family and known as stevia, sweet leaf, honey leaf, and candy leaf.
Twentyfour hydroxycinnamic acid derivatives of quinic and shikimic acid were detected, and 19 of them were successfully. Effectiveness of stevia rebaudiana whole leaf extract against. Stevia stevia rebaudiana is a bushy shrub that is native to northeast paraguay, brazil and argentina. Stevia rebaudiana bertoni is a medicinal plants and commercially use as non caloric sweetener for diabetic patient. In vitro callusing in stevia rebaudiana bertoni using cyanobacterial mediaa novel approach to tissue culture. Bertoni pourvi jain, sumita kachhwaha and s l kothari department of botany, university of rajasthan, jaipur 302 004, india received 29 october 2011. Stevia rebaudiana has been used in numerous countries for thousands of years as a source of sweetener that is all natural and will not raise a persons glycemic index, which is. It has elongated leaves that grow along the stems and are lined up against each other. Nov 12, 2015 considering the effectiveness of stevia leaf extract in laboratory and clinical studies, we evaluated the antimicrobial potential of stevia whole leaf extracts against the lyme disease causing pathogen, b.
Stevia rebaudiana leaves were obtained from anna medicinal farm, chennai. In vitro antioxidant activities of stevia rebaudiana. Uses, side effects, interactions, dosage, and warning. In the present study, a protocol was developed for in vitro micropropagation using 6benzylamino purine bap and kinetin kn for the formation of multiple shoot proliferation and indole3acetic acid iaa, indole3butyric acid iba and 1naphthaleneacetic acid. Around 82% of plants were successfully established in natural field condition.
It is now grown in other parts of the world, including canada and part of asia and europe. Leaf extract of stevia rebaudiana promotes effects on certain physiological systems such as the cardiovascular and renal and influences hypertension and hyperglycemia. Nutramedix stevia was identified as stevia a in a study published in 2015 where it was shown to be more effective than doxycycline in eliminating. Response surface methodology was used to design experiments by varying three factors. Pdf in vitro micropropagation of stevia rebaudiana bert. Influence of nacl on biochemical parameters of two cultivars of stevia rebaudiana regenerated in vitro. The leaves of stevia are the source of sweet glycosides.
Stevia rebaudiana bertoni, known to be an antidiabetic medicinal herb, belongs to asteraceae family which has bioactive compounds stevioside and rebaudioside in its leaves that taste about 300350 times sweeter than sucrose. Antioxidants free fulltext antioxidant metabolism and. An efficient stevia rebaudiana transformation system and in. Stevia rebaudiana bertoni is a medicinal plant belongs to asteraceae family and known as stevia, sweet leaf, honey leaf, and. Effectiveness of stevia rebaudiana whole leaf extract. Optimization of micronutrients for the improvement of in vitro plant regeneration of stevia rebaudiana bert. An efficient plant regeneration protocol has been developed from leaf explants of stevia rebaudiana, an economically and medicinally important. In vitro culture studies on stevia rebaudiana request pdf.
Morphoanatomical study of stevia rebaudiana roots grown in. Stevia rebaudiana bertoni plants grown in vitro and ex vitro were investigated for variations in the profile of steviosides in their leaves, shoots, roots and flowers. Pdf in vitro propagation of stevia rebaudiana bertoni. Impact of glycine betaine on salinity tolerance of stevia. Influence of nacl on biochemical parameters of two. Stevia rebaudiana bertoni is a medicinal plants and commercially use as noncaloric sweetener for diabetic patient. Mutagenicity and human chromosomal effect of stevioside, a. Family asteraceae is a natural sweetener plant that is grown commercially in many parts of brazil, paraguay, central america, thailand, korea, china and india. Lingstrom b, d a department of health sciences, and b who collaborating centre for epidemiology and community dentistry. Preparation of plant extract 25 g of airdried powder of stevia rebaudiana leaves was immersed in 100 ml of organic. In vitro culture studies on stevia rebaudiana springerlink.
It is commonly known as candyleaf, sweetleaf or sugarleaf it is a small seasonal plant which grows to a height of 12 feet 3061 cm. In vitro propagation of stevia rebaudiana bertoni in egypt. For invitro regeneration of stevia rebaudiana bertoni murashige and skoogs medium was used with various concentrations of plant growth regulators7. This plant has been used in folk medicine to treat hypertension, diabetes and as a contraceptive.
Effect of different levels of farmyard manure and nitrogen on the yield and nitrogen uptake by stevia stevia rebaudiana bertoni. Stevia rebaudiana, bertoni is a small shrub belong to family. Stevia rebaudiana bertoni is one of a very few plant species that produce zero calorie, sweet compounds known as steviol glycosides sg. Protocol optimization for efficient in vitro micro. Stevia rebaudiana extract is often referred to as stevia. Comparative studies of in vitro and in vivo grown plants.
Invitro antimicrobial and antitumor activities of stevia. An efficient high frequency plant regeneration protocol through direct organogenesis was developed for sevia rebaudiana. Antioxidant metabolism and chlorophyll fluorescence during. Materials and methods plant material stevia rebaudiana plants were collected from horticultural institute, yercaud, tamilnadu, india and maintained in green house.
Two node microcuttings were subjected to ms media supplemented with different nacl concentrations 0, 25, 50, 75, 100, 125 mm. Stevia rebaudiana bertoni, belonging to family asteraceae and natural sweet plant, but due to poor seed viability, fertility and vigor, stevia cultivation is a challenging task. It is a small seasonal plant which grows to a height of 12 feet 3061 cm. Pdf micropropagation of stevia rebaudiana in vitro researchgate. Shoot apex, nodal, and leaf explants of stevia rebaudiana bertoni can regenerate shoots when cultured on murashige and skoog ms medium supplemented with 6benzyladenine ba. Qualitative composition of the sgs in in vitro plants was found to be identical to that of intact plants, but their content in the former plants appeared to be about five or six times lower.
Studies on rapid micropropagation of stevia rebaudiana. Stevia is sold as a concentrated white stevia stevia rebaudiana powder to sprinkle on food. Leaves of stevia rebaudiana bertoni have been popularly used as a sweetener in foods and beverages for diabetics and obese people due to their potent sweetener stevioside. Stevia rebaudiana bertoni is a small perennial shrub of the asteraceae compositae family that is native to paraguay, brazil, and argentina.
The extract is about times sweeter than sugar so it can be used at a very low concentration. A survival rate of 70% was recorded at the hardening phase on the. The present study was aimed at the developing an efficient protocol for successful in vitro rooting of stevia shoot fragments and subsequent acclimatization of. In vitro culture is a technique that allows studying the behavior of agnps in plants of agricultural interest such as stevia stevia rebaudiana b. In vitro shoot multiplication in stevia rebaudiana bert. The protocol for in vitro propagation of stevia rebaudiana is scanty sivaram and mukundan, 2003. Stevia is an extract from stevia rebaudiana, having up to 300 times the sweetness of sugar. An in vitro and in vivo comparison of the effect of stevia. In vitro shoot multiplication of stevia rebaudiana, an important plant with high economic and medicinal values. Shoot tips containing axillary buds were used as an explant and inoculated on murashige and skoogs ms medium containing 3%. Stevia also suitable to be cultivated in semiarid climates and coastal areas, which are characterized by the low quality of the irrigation water. In vitro propagation is important for rapid multiplication of a wide range of nursery crops or medicinal plants, including stevia stevia rebaudiana bertoni. Introduction stevia rebaudiana bert belonging to the family compositae, is one of the most valuable tropical medicinal plant. For invitro regeneration of stevia rebaudiana bertoni murashige and skoogs medium was used with various concentrations of plant growth regulators 7.
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